Selective Toxicity of Non Polar Bioactive Compounds of Persian Gulf Sea Squirt Phallusia Nigra on Skin Mitochondria Isolated from Rat Model of Melanoma
نویسندگان
چکیده
Background: Skin cancer is the most prevalent cancer and one of the major causes of mortality worldwide. Marin animals have attracted much attention in recent years as useful substances having application in medicine. It was shown that Phallusia nigra (P. nigra) known as sea squirt could play an important role in cancer therapy. Methods: This study was designed to figure out the probable selective toxicity of n-hexane, diethyl ether, methanolic and aqueous extracts of P. nigra on cancerous mitochondria isolated from the skin of melanoma induced rats. In our study, mitochondria were isolated from the skin tissue of both melanoma induced and normal healthyrats. Different concentrations of four different extracts of P. nigra (250, 500 and 1000 μg/ml) were added to mitochondrial samples obtained from both groups, separately. Results: Our results showed that n-hexane, diethyl ether and methanolic extracts (but not aqueous extract) of P. nigra in all concentrations applied (250, 500 and 1000 μg/ml) significantly induced toxic alterations only in the cancerous but not normal healthy skin mitochondria including; increased reactive oxygen species (ROS) formation, mitochondrial swelling, decreased mitochondrial membrane potential (MMP) and cytochrome c release. Flow-cytometry analysis demonstrated that n-hexane, diethyl ether and methanolic extracts of P. nigra progressively induced apoptosis and necrosis only on melanoma cells but not healthy skin cells. Conclusions: Our results suggest that non polar bioactive compounds in P. nigra may be hopeful candidates for further studies including molecular identification, confirmatory in vivo experiments and finally clinical trials designed for new drug treatment of melanoma skin cancer.
منابع مشابه
A search for anti-carcinogenic and cytotoxic effects of Persian Gulf sea snake (Enhydrina schistosa) venom on hepatocellular carcinoma using mitochondria isolated from liver
Common techniques for the treatment of Hepatocellular carcinoma (HCC) have not been successful, and thus the design and discovery of new compounds with better anti-cancer function are needed. Snake venom is among the most important compounds used by researchers to the treatment of various cancers. This study was designed to evaluate the toxicity effect of Persian Gulf snake venom (Enhydrina sch...
متن کاملAntibacterial Activity of Tunichrome Released from phallusia nigra Marine Tunicate Obtained from Bushehr Coast
Background: Marine life has long been used for their bioactive compounds. Several amazing compounds with different biological activities have so far been identified in tunicates. The aim of the current study was to evaluate the antimicrobial activity of tunichrome released from phallusia nigra marine tunicates collected from Bushehr province coasts, against four strains of Staphylococcus aure...
متن کاملSelective Toxicity of Persian Gulf Sea Cucumber (Holothuria parva) and Sponge (Haliclona oculata) Methanolic Extracts on Liver Mitochondria Isolated from an Animal Model of Hepatocellular Carcinoma.
BACKGROUND Natural products isolated from marine environments are well known for their pharmacodynamic potential in diverse disease treatments, such as for cancer or inflammatory conditions. Sea cucumbers are marine animals of the phylum Echinoderm and the class Holothuroidea, with leathery skin and gelatinous bodies. Sponges are important components of Persian Gulf animal communities, and the ...
متن کاملA Comparison of Toxicity Mechanisms of Cigarette Smoke on Isolated Mitochondria Obtained from Rat Liver and Skin
Previous studies demonstrated that CSE induces oxidative stress and its consequences on isolated mitochondria obtained from lung, heart and brain which may provide insight into the role of CSE in human health and disease. The present study was carried out to further characterize and compare toxic effect of CSE extract on isolated mitochondria obtained from either a directly contacting tissue (i...
متن کاملA Comparison of Toxicity Mechanisms of Cigarette Smoke on Isolated Mitochondria Obtained from Rat Liver and Skin
Previous studies demonstrated that CSE induces oxidative stress and its consequences on isolated mitochondria obtained from lung, heart and brain which may provide insight into the role of CSE in human health and disease. The present study was carried out to further characterize and compare toxic effect of CSE extract on isolated mitochondria obtained from either a directly contacting tissue (i...
متن کامل